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1). Efficacy: Powerful Oral Antimicrobial: Stannous Fluoride is an antimicrobial that destroys a broad spectrum of pathogens - including bacteria, viruses and fungi.

2). Safety: It is a prescriptive drug - unless delivered in unit doses that preclude ingestion of sufficient quantities to be toxic. - as ONLY MedActive's rinse is.

3). Protection for others: Use of MedActive Oral Rinse protects against the TRANSMISSION of pathogens from an infected individual to the surrounding environment/people by destroying infectious pathogens in the oral cavity.

4). Protection for users: MedActive Oral Rinse destroys bacteria in the mouth and supports/strengthens the body's natural defense (immune) system. This is especially important/beneficial for persons "at-risk" from immuno-compromising conditions and diseases and for individuals deemed as working in "High-Risk" environments.

5). Ease of Use: The unit dose dispenser provides protection from cross contamination, over dosing and under-dosing, maximum portability, disposability and multiple uses as needed.

6). Substantivity (long lasting): The rinse provides ongoing protection from infection for up to 6 hours per use.

• 1). Efficacy: Powerful Oral Antimicrobial: Stannous Fluoride is an antimicrobial that destroys a broad spectrum of pathogens - including bacteria, viruses and fungi.
• 2). Safety: It is a prescriptive drug - unless delivered in unit doses that preclude ingestion of sufficient quantities to be toxic. - as ONLY MedActive's rinse is.
• 3). Protection for others: Use of MedActive Oral Rinse protects against the TRANSMISSION of pathogens from an infected individual to the surrounding environment/people by destroying infectious pathogens in the oral cavity.
• 4). Protection for users: MedActive Oral Rinse destroys bacteria in the mouth and supports/strengthens the body's natural defense (immune) system. This is especially important/beneficial for persons "at-risk" from immuno-compromising conditions and diseases and for individuals deemed as working in "High-Risk" environments.
• 5). Ease of Use: The unit dose dispenser provides protection from cross contamination, over dosing and under-dosing, maximum portability, disposability and multiple uses as needed.
• 6). Substantivity (long lasting): The rinse provides ongoing protection from infection for up to 6 hours per use.

MEDACTIVE ORAL ANTIMICROBIAL RINSE WITH ULTRAMULISON AND SPILANTHOL    The recent outbreak of Swine Flu in Mexico has now spread across the border into the United States. People who are classified as being “High Risk” populations should use every means at their disposal to protect themselves from this deadly virus. MedActive Antimicrobial Rinse is NOT a viral vaccine and we urge all concerned persons to follow the CDC and WHO prevention guidelines.    MedActive Antimicrobial 0.63% Stannous Fluoride Rinse is a powerful anti-infection agent that provides an additional layer of protection against aerosol born pathogens that can travel thru the air, into the mouth and then be swallowed into the respiratory system. The rinse is provided in Patient-Friendly single use dispensers that deliver optimal antimicrobial bioavailability and eliminate the risk of cross contamination. The rinse is a highly effective protection against Streptococcal bacteria and Streptococcus Pneumoniae and serves to enhance and support the mouths natural immune system. Persons who are at risk of inhaling infectious bacteria thru close contact to people in the work place or while traveling can gain up to 8 hours of oral protection from each rinse.    Aerosols:  There is a long history of infections that have been transmitted by an airborne route. 1 More common is the apparent spread of cold and influenza viruses by airborne routes. However, the actual documentation of an airborne route for transmission of cold and influenza viruses is difficult to verify. Because cold and flu viruses can be transmitted by contact, contaminated objects and an airborne route, in a flu outbreak it is often difficult to know the exact route by which the virus is transferred.²  SARS recently has been reported in China, Canada, and other countries.  The exact mechanisms by which SARS is spread remains uncertain, but it is clear that the primary method is through aerosolized droplets produced by coughing. ³  The Centers for Disease Control and Prevention, or CDC, and the ADA have recommended that aerosol-producing procedures should be avoided in patients with active SARS.4  The oral environment is inherently wet with saliva that continuously replenishes the fluid in the mouth. The fluids in the mouth are grossly contaminated with bacteria and viruses. 5  The saliva and nasopharyngeal secretions also may contain other pathogenic organisms. These may include common cold and influenza viruses, herpes viruses, pathogenic streptococci and staphylococci, and the SARS virus. 6    


   With the recognition that overall aerosols are a potent source of infectious agents, Universal precaution to limit aerosols should be used.   One method of reducing overall bacterial counts produced during dental procedures is the use of a preprocedural rinse. The use of a .01 percent chlorhexidine mouthwash for one minute before a dental procedure has been shown to significantly reduce the bacterial count in the air of the operatory.” Chlorhexidine is an effective antiseptic for free-floating oral bacteria such as those found in the saliva and those loosely adhering to mucus mem-branes. Chlorhexidine, however, does not affect bacteria in a biofilm such as established dental plaque. 7 See:      S.K. Harrel and J.Molinar. “Aerosols and splatter in dentistry, A brief review of the literature    and infection control implications” JADA Vol 135, April 2004.     No scientific evidence indicates that preprocedural mouth rinsing prevents clinical infections among patient or dental health care personnel. However, studies have shown that a preprocedural rinse with a product containing an antimicrobial product can reduce the level of oral microorganisms generated in aerosols. 8, 9  See:  CDC.gov/ORALHEALTH/Infection Control and USAF, “Infection Control in Dental Settings”   Preprocedural Mouth Rinse.      Stannous Fluoride vs Sodium Fluoride  The antibacterial properties of a 0.50% stannous fluoride mouthwash and a 0.27% sodium fluoride mouthwash of equivalent fluoride ion concentration (0.12%) were examined using standard bacteriologic plate count procedures. The stannous fluoride solution produced a significant bacterial effect, but the bactericidal activity of the sodium fluoride solution was negligible. 10 These data indicate that a 0.50% stannous fluoride solution produces a highly significant bactericidal effect when used as a mouthwash. 11   See:    C Andres, J. C. Schaefer & A. S. Windeler, Jr, “Comparison of Antibacterial Properties of   Stannous Fluoride and Sodium Fluoride Mouthwashes”: J Dent Res 1974; 53:457              Biofilm  

   With the recognition that overall aerosols are a potent source of infectious agents, Universal precaution to limit aerosols should be used.   One method of reducing overall bacterial counts produced during dental procedures is the use of a preprocedural rinse. The use of a .01 percent chlorhexidine mouthwash for one minute before a dental procedure has been shown to significantly reduce the bacterial count in the air of the operatory.” Chlorhexidine is an effective antiseptic for free-floating oral bacteria such as those found in the saliva and those loosely adhering to mucus mem-branes. Chlorhexidine, however, does not affect bacteria in a biofilm such as established dental plaque. 7 See:      S.K. Harrel and J.Molinar. “Aerosols and splatter in dentistry, A brief review of the literature    and infection control implications” JADA Vol 135, April 2004.     No scientific evidence indicates that preprocedural mouth rinsing prevents clinical infections among patient or dental health care personnel. However, studies have shown that a preprocedural rinse with a product containing an antimicrobial product can reduce the level of oral microorganisms generated in aerosols. 8, 9  See:  CDC.gov/ORALHEALTH/Infection Control and USAF, “Infection Control in Dental Settings”   Preprocedural Mouth Rinse.      Stannous Fluoride vs Sodium Fluoride  The antibacterial properties of a 0.50% stannous fluoride mouthwash and a 0.27% sodium fluoride mouthwash of equivalent fluoride ion concentration (0.12%) were examined using standard bacteriologic plate count procedures. The stannous fluoride solution produced a significant bacterial effect, but the bactericidal activity of the sodium fluoride solution was negligible. 10 These data indicate that a 0.50% stannous fluoride solution produces a highly significant bactericidal effect when used as a mouthwash. 11   See:    C Andres, J. C. Schaefer & A. S. Windeler, Jr, “Comparison of Antibacterial Properties of   Stannous Fluoride and Sodium Fluoride Mouthwashes”: J Dent Res 1974; 53:457              Biofilm  


See:   H.]. BUSSCHERI, Di. WHITE 2, G.I. GEERTSEMA-DOORNBUSCH, 3. ATEMA-SMIT, and H.C. VAN DER MEI, University Medical Center Groningen and University of Groningen, Netherlands, The Procter and Gamble Company, Mason, OH, USA; “2009 Stannous Fluoride Reduces EPS Production by Oral Biofilm Bacteria”      Antifungal  In vitro antifungal effect of amine fluoride-stannous fluoride combination on Oral Candida species. 14

Antifungal  In vitro antifungal effect of amine fluoride-stannous fluoride combination on Oral Candida species. 14  
Conclusion:AmF/SnF2 could be used as a potent adjunct to antifungal therapy for oral yeasts. Although different Candida species demonstrated variable sensitivity the most prevalent oral yeast C. albicans appeared sensitive to the AmF/SnF2 combination.  

JH Meurman, K. Kari, T Waltimo, A. Kotiranta, J. Inken, LP Samaranayake: “In vitro antifungal effect of  amine fluoride-stannous fluoride combination on Oral Candida species”; Oral Disease Vol 12 Iss.1;  2005; 45-50.    Why Stannous Fluoride  In 1980, Tinanoff and colleagues studied the effectiveness of a twice-daily 0.1 % stannous fluoride mouthrinse on plaque formation-when compared with a placebo rinse, using double blind crossover design. They observed statistically significant (50.2%) reductions in total colony forming units in the stannous fluoride groups. 15

First, unlike other fluoride agents (sodium monofluorophosphate and sodium fluoride) stannous fluoride exhibits intrinsic antimicrobial effects. These effects, in fact, appear stronger than those observed for numerous other antimicrobial agents, most notably, essential oils and triclosan. Second, since stannous fluoride is a proven active anticaries ingredient, its use as an antimicrobial does not require added stabilization with a separate fluoride source. Third, stannous fluoride had previously exhibited clinical efficacy for gingivitis control when properly stabilized in the form of anhydrous gels. Finally, stannous fluoride exhibits considerable reactivity with root surfaces, and has been clinically shown to produce reductions in dentinal hypersensitivity, a common ailment of the middle aged. Practically speaking, stannous fluoride has been, and continues to be, an “all-in-one” active therapeutic ingredient. 16  See:   Jorin Karch; Diagnosing and Managing the Periodontal Patient; “Stannous Fluoride in Periodontal   Maintenance” Clinical Dentistry 20 Vol 3, Chapter 14.

     Spilanthes Herb Profile  Also known as Spilanthes acmella, Toothache Plant, and Paracress  Spilanthes is commonly called the toothache plant * and with good reason. The leaves and flower heads, particularly the young buds, contain a natural analgesic which numbs the tongue and gums when chewed, thus relieving the pain of toothaches. It also stimulates the salivary glands to produce more saliva, and may function as a simple tonic for healthy gums and oral flora. But that’s only the start of the useful properties of spilanthes. The main constituent of spilanthes, spilanthol, is an effective antiparasitic, and has been used as a native remedy against malaria. It has natural antibacterial and antifungal actions as well, making it a good all purpose purifier. It seems to boost production of leukocytes and antiviral interferon, suggesting that the plant may be useful in enhancing immune system function, and it is often used topically to treat bacterial infection of the skin and wounds, and fungal infections like ringworm.  Constituents spilanthol, stigmasterol, stig masteryl-3-O-b-D-glucopyranoside, triterpens   From: http:/Iwww.mountainroseherbs.com/Iearn/spilanthes.pI Leaves, buds, flowers and stems  Typical Preparations Buds and leaves may be chewed directly, or made into a water extract. Sometimes found in an alcohol extract.  Summary Spilanthes is surprisingly unknown for a plant that has such a specific effect. The analgesic and antibiotic properties of spilanthes are well-known to scientists, and its efficacy against malaria strongly suggests that it may have a similar effect against other blood parasites such as Lyme disease. A mouth rinse of spilanthes extract can be used daily to promote gum health, and chewing as little as a single bud of the plant can numb the mouth and reduce the pain of toothache for up to 20 minutes depending on the sensitivity of the person. The most promising research into the use of spilanthes, though, is in its antibacterial properties. So far, in vitro testing has shown that the plant’s extract has strong effect against E.coli, pseudomonas, salmonella, klebsiella pneumonae and staphylococcus albus, as well as inhibiting the growth of candida albicans.    Precautions   None known 17            

   SPILANTHOL, A NITRIC OXIDE SYNTHASE INHIBITOR   Inflammatory Responses Inflammatory responses, initiated by the invasion of pathogens or by tissue injury, comprise a series of vascular and cellular reactions. The processes come with nonspecific cellular responses which generate chemical messages through two mediators: (a) cell-derived, and (2) plasma-derived.  Cell derived mediators are produced by white blood cells and include:  arachidonic acid derivatives (prostaglandins and leukotrines) cytokines lymphokines  monikines  platelet activating factors  histamines  the kiwin system  Among the cellular responses, the activation of macrophages is essential to the initiation and continuation of defensive reactions.  Once they are stimulated by pathogens, macrophages yield:  nitric oxide (NO),  prostaglandins E2 (PGE2),  interleukins IL-1, IL-6 and IL-12, and other pro-inflammatory cytokines to enhance the defense capacity.  See:  Dinarello. “Interleukin- 1, interleukin- 1 receptors and interleukin- 1 receptor-antagonist.” Int. Rev. Immunol. 1998, 16, 457-499.  Erwig and Rees. “Macrophage Activation and programming and its role for macrophage function in glomerular inflammation.” Kidney Blood Press Res. 1999, 22, 21-25.  Nathan. “Natural resistance and nitric oxide.” Cell 1995, 82, 873-876.  Chronic exposure to high levels of NO frequently results in inflammatory diseases. Most NO is produced from three nitric oxide syntheses in the mammalian cells. The inducible NOS form (iNOS) catalyzes the formation of the majority of NO and is present in:  neutrophils,  macrophages,  smooth muscle cells, and  endothelial cells.

 
   SPILANTHOL, A NITRIC OXIDE SYNTHASE INHIBITOR   Inflammatory Responses Inflammatory responses, initiated by the invasion of pathogens or by tissue injury, comprise a series of vascular and cellular reactions. The processes come with nonspecific cellular responses which generate chemical messages through two mediators: (a) cell-derived, and (2) plasma-derived.  Cell derived mediators are produced by white blood cells and include:  arachidonic acid derivatives (prostaglandins and leukotrines) cytokines lymphokines  monikines  platelet activating factors  histamines  the kiwin system  Among the cellular responses, the activation of macrophages is essential to the initiation and continuation of defensive reactions.  Once they are stimulated by pathogens, macrophages yield:  nitric oxide (NO),  prostaglandins E2 (PGE2),  interleukins IL-1, IL-6 and IL-12, and other pro-inflammatory cytokines to enhance the defense capacity.  See:  Dinarello. “Interleukin- 1, interleukin- 1 receptors and interleukin- 1 receptor-antagonist.” Int. Rev. Immunol. 1998, 16, 457-499.  Erwig and Rees. “Macrophage Activation and programming and its role for macrophage function in glomerular inflammation.” Kidney Blood Press Res. 1999, 22, 21-25.  Nathan. “Natural resistance and nitric oxide.” Cell 1995, 82, 873-876.  Chronic exposure to high levels of NO frequently results in inflammatory diseases. Most NO is produced from three nitric oxide syntheses in the mammalian cells. The inducible NOS form (iNOS) catalyzes the formation of the majority of NO and is present in:  neutrophils,  macrophages,  smooth muscle cells, and  endothelial cells.


    Nitric Oxide Synthase  The relevance of NO as a mediator of inflammation was reported by DiRosa, et. al. Prostaglandins, Leukotrienes Essential Fatty Acids 1996; 54:229.  Inflammation is a complex process characterized by the contributors of several mediators, including: prostaglandins (PGs) and nitric oxide (NO). See Higgs, et. al. Ann. Clin. Res. 1984; 16:287, and Nathan Clin. Invest. 1997; 100:2417.  The central role of PGs in inflammation was firmly established since the discovery that the anti-inflammatory action of the ASA-like drugs was mediated by the inhibition of the enzyme cyclooxygenase (COX) which controls arachodonic acid to PGs. See:  Vane Nat. New Biol. 1971; 231-232, and  Manacada Nature 1976; 263 :663.  Blantz & Munger in Nephron 2002; 90, 373-378, describe the “Role of nitric oxide in inflammatory conditions” as follows:  Nitric Oxide (NO) plays an important regulator/modulatory role in a variety of inflammatory conditions.  NO is a small, short-lived molecule that is released from a variety of cells in reponse to homeostatic and pathologic stimuli.  Nitric Oxide is synthesized from the amino acid L-arginine by NO synthase (NOS).  NO produced from iNOS is probably more important in host defense and chronic inflammatory responses. See Nossler and Billiar J. Leukoe Bio. 1993; 54:171-178.  Thiemermann and Vane in Eur. J. Pharmacol. 1990; 182, 591-595, describe that the inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharide in rats.  DeGroote and Fang in Clin. Infect. Dis. 1995; 21 (Suppl. 2): 162-165, resports on NO inhibitions: Antimicrobial properties of Nitric Oxide.  Chen, et. al., in Biochemical Pharmacology 2000; Vol. 59, 1445-1457, report:  “induced iNOS catalyzes the formation and release of a large amount of NO, which plays a key role in the pathophysiology of a variety of diseases.... NO production catalyzed by iNOS, therefore, may reflect the degree of inflammation and provides a measure by which effects of drugs on the inflammatory process can be assessed. Expression of COX-2 in various tissue preparations following lipopolysaccharide treatment also has been reported. This enzyme is considered to play a major role in the inflammatory process by catalyzing the production of prostaglandins....”

 NF-ĸB is a transcription factor that is activated in response to stimulation by LPS, and activation of NF- ĸB  is an essential step in inducing iNOS gene expression in macrophages. See: Kim, et. al. Biochem. Biophysics Res. Commun. 1997; 236:655-660.  In summary, results of the present study indicated that oroxylin A was an effective inhibitor of LPS induced iNOS and COX-2 gene expression by blocking NF-B activation in RAN264.7 macrophages.  L. Santebin in Fitoterapia 2000, 71;S48-S57, reports: “The need for compounds which selectively inhibit the inducible isoforms of cyclooxygenase (COX-2) and/or nitric oxide synthase (iNOS enzymes), which play an important role in the pathophysiology of the inflammatory process.... has prompted several research groups to screen medicinal plants traditionally used as inflammatory agents.”  The authors continue: “Nitric oxide synthase the enzyme which converts L arginine to nitric oxide, exists in two isoforms. It appears that the constitutive isoforms of both enzymes (cyclooxygenase- 1 and constitutive nitric oxide synthase) have a regulatory-physiological role, whereas the inducible isoforms (cyclooxygenase-2 and inducible nitric oxide synthase) are involved in inflammation. A number of medicinal plants have been screened for their ability to inhibit cyclooxygenase-2 and/or inducible nitric oxide synthase activity and/or expression.” The author also identifies prostaglandins and nitric oxide as molecular targets for autoinflammatory therapy.  The author concludes: “Traditional medicine is a valuable source for the development of new, effective, anti-inflammatory, therapeutic agents, especially in the light of the new findings concerning the role of PGs and NO in inflammation. Thus, although the drugs presently used for anti-inflammatory therapy are still NSAIDs, other emerging, possible strategies, such as iNOS or COX-2 inhibitors, as well as the inhibition of the formation of reactive nitrogen species should be carefully evaluated.”  Periodontitis is the most frequent cause of tooth loss in adults. Nitric Oxide Synthase (NOS) has been linked to bone resorption mechanisms during periodontitis inflammation:  (1) A study reported by R.F.C. Leitao, et. al. in Journal of Periodontology, June 2005, Vol. 76, No. 6, 956-963, investigated the effect of NOS (nitric oxide synthase) inhibitors in the alveolar bone loss in an experimental periodontitis disease (EPD) model. Data reported provided evidence that NOS inhibitors prevent inflammatory bone resorption in experimental periodontitis.  (2) In a later study by Leitao, et. al., in 2007, Cancer Chemotherapy & Pharmacology 59.5, 603, the role of nitric oxide on pathogenesis of 5-fluororacil induced experimental oral mucositis in hamsters was reported.  (3) Kim Hurl and Sayden in Science 23 Dec. 2005, Vol. 310, No. 5756, 1966-1970 reported: Inducible Nitric Oxide Synthase binds, S-nitrosylates and activates cyclo oxygenase-2 (COX-2). COX-2 and nitric oxide synthase are two major inflammatory mediators. NOS specifically binds to COX-2 disrupting NOS-COX-2 binding prevented NO-mediated activation of COX-2.

  (4) Suppression of inducible nitric oxide synthase and cyclooxygenase-2 by garcinol is reported by Liao, et. al. in Mol. Carcinogenesis 2004, 41, 140-149.  (5) Blantz and Munger describe the role of nitric oxide in inflammatory conditions in Nephron 2002, 90, 373-378. “The L-arginine: nitric oxide pathway in the colonic epithelium is discussed and illustrated. In normal condition with physiological intracellular concentrations of L-arginine, nitric oxide synthase (NOS) produces nitric oxide (NO). In inflammation, NOS activity is enhanced, which is proposed to cause L-arginine depletion and lead to a disruption of electron transport within the enzyme so that superoxide (02) rather than NO is formed by iNOS. Due to the free radcial nature of NO and O2, the generation of peroxynitrite (OONO) occurs instantaneously and is the cause of cellular damage. This substrate dependent dual action of NOS is proposed to be of clinical significance in severe colonic inflammation.  At the present time, there is much interest in the potential therapeutic properties of L-arginine and inhibitors of NOS in modulating NO bioavailability in the various conditions associated with inflammation.”  (6) Perner and Rask-Madsen in a review article describe the potential role of nitric oxide in chronic inflammatory bowel disorders. Aliment Pharmacol. Ther. 1999, 13, 135- 144.  (7) Christopherson and Bredt describe the role of nitric oxide in excitable tissues: physiological roles and disease. J. Clin. Invest. 1997, 100, 2424-2429.  (8) Papertropoulos, et. al. describe molecular control of nitric oxide synthase in the cardiovascular system in Cardio Vas. Res. 1999, 43, 509-520.  (9) Kirkwood, et. al. Periodontology 2000; 43:1, 294 discuss novel host response therapeutic approaches to treat periodontal diseases.  (10) Chen, et. al. report on new insights into the role of nuclear factor Kappa B, a ubiquitous transcription factor in the initiation of diseases. Clin. Chem. 1999, 45, 7-17.

  Spilanthes acmella  Spilanthes acmella, a common spice, has been administered as a traditional folk medicine for years to cure: toothaches, stammering and stomatitis. The flowers and leaves of S. acmella have a pungent taste and cause tingling and numbness. Spilanthol, an olefinic alkamide with an isobutyl side chain is the main constituent in flowers of S. acmella. Several studies have demonstrated S. acmella: diuretic, analgesic, antibacterial, anti-inflammatory and saliva stimulator activities.  See:  Ramsewak, et. al. Physiochemistry 1999, 732, 9  Ratnasooriya, et. al. J. Ethnopharmaco 2004, 97, 317-320  Holetz, et. al. Mem. Inst. Oswaldo, Cruz 2002, 97, 1027-103 1  Leal-Cardoso, Fonteles. An. Acad. Bras. Ciena 1999, 71, 207-213  Rojas, et. al. BMC Complement Altern. Med. 2006, 6, 2-7  Ley, et. al. Nat. Prod. Res. 2006, 20, 798-804  Nakatami and Nagashima. Brosci Biotechnol. Biochem. 1992, 56, 759-762   Wu, et. al. J. Agric. Food Chem. 2008, 2341-2349, reported:  “This study has isolated the anti-inflammatory bio-active compound, spilanthol, demonstrating significant suppression effects on inflammatory response. This is the first report to identify the anti-inflammatory compound, spilanthol, of s. acme/la.”  This study isolates the active compound, spilanthol, by a bioactivity-guided approach and indicates significant anti-inflammatory activity on lipopolysaccharide-activated murine macrophage model RAW 264.7.  Analytical results revealed that spilanthol isolated from chloroform extracts, efficiently down-regulated the production of inflammatory mediators IL-1B, IL-6 and TNF-a and attenuated the expression of COX-2 iNOS. The inhibitory effect could be partly due to the reduced activation of NF-B. Moreover, the extracts of S. acmella demonstrated radical scavenging activity, which could reduce cell and tissue damage caused by free radicals.  The authors conclude: “Chronic exposure to high levels of NO frequently results in inflammatory diseases.”

   “Inhibitory Effect of Spilanthol on LPS-induced NO.... The addition of spilanthol reduced the degree of cell spreading and pseudopodia formation.... revealing the suppression of cell activation.  Inhibitory Effect of Spilanthol LPS-induced iNOS and COX-2 mRnA Production    These results indicate that the administration of spilanthol restrained iNOS and COX-2 production at the transcriptional and translational levels.  Inhibitory Effects of Spilanthol on LPS-induced Pro-inflammatory Cytokine Production  These results indicate that spilanthol substantially reduced the production of pro-inflammatory cytokines, IL-1B, IL-6 and TNF-2.  Effect of Spilanthol on LPS-induced NF-KB Activation   These results show that the reduced levels of phosphorylated 1-KB could increase the amount of NF-KB/IKB complexes in the cytoplasma, in turn limiting the translocation of NF-KB to the nucleus to transcribe inflammatory mediators.  Radical Scavenging Activity of Extracts.... These results suggested that these extracts, particularly the EA fraction, served as free radical scavengers and were likely to lower the inflammatory responses caused by free radicals.”  The authors conclude: “These results indicate that the administration of spilanthol restrained iNOS and COX-2 production at the transcriptional and translational levels.” Additionally, the LPS-stimulated IL-1B, IL-6 and TNF-2 productions are dose-dependently reduced by spilanthol.  This study indicates that spilanthol markedly reduces the production of inflammatory cytokines (IL-1B, IL-6, TNF- a) in LPS-treated murine RAW 264.7 macrophages which could possibly ameliorate the inflammation. Additionally, spilanthol also inhibits the expression of COX-2, reducing the progression of inflammatory responses. These suppression effects are partly due to the inactivation of NF-KB. Results reveal the spilanthol dose-dependently inhibits the phosphorylation of IxB in cytoplasm resulting in the possible accumulation of NF-KB/IKB complex in a cytoplasm. Unsurprisingly, the p65 translocation was impeded by spilanthol, indicating that the NF-KB activation was limited. The production of pro-inflammatory cytokines and the expressions of other inflammatory mediators such as NO, iNOS and COX-2 were thus reduced.              


 Summary  A long history of infections being transmitted via the oral fluids and aerosols exist.  Universal precautions to avoid the spread of infections are needed.  Preprocedural rinsing literature demonstrates significant reductions in aerosols.   Stannous Fluorides has been evaluated at length and demonstrates the ability to be both broad spectrum in nature and duration of effect up to 12 hours.  In fact, it is considered a therapeutic all-in-one agent.  Spilanthol is a natural plant  which, demonstrates antibacterial, antifungal actions as well as being a regulator of TNF2 function.  In Conclusion:  MedActive Oral Stannous Fluoride Rinse with Spilanthol demonstrates the ability to provide protection for the spread of aerosols related to a broad host of infectious agents, including Bacteria, yeast, viruses and fungal agents.  Jeffrey A. Hameroff, D.D.S. Clinical Director MedActive Oral Pharmaceutics